CEA Direction des sciences du vivant - iRTSV : Spectroscopie Mössbauer

Spectroscopie Mössbauer

Personnel de l'équipe impliqué dans le projet

Martin Clémancey, Ingénieur d'études UJF
Geneviève Blondin, Chargée de Recherche CNRS
Ricardo Garcia, Maître de Conférences Université Joseph Fourier
Jean-Marc Latour, Directeur de recherche CEA

The team Physical chemistry of Metals in Biology from the Laboratory of Chemistry and Biology of Metals develops the Mössbauer spectroscopy platform dedicated to iron proteins and their synthetic analogs.
Mössbauer spectroscopy is a magnetic technique that allows the study of ca forty nuclei among which iron, which makes it especially attractive for biological applications. M¨ssbauer spectroscopy detects all the iron contained in a sample whatever its chemical form or its spin state and detects exclusively iron.

Apparatus
LCBM Mössbauer platform is the only one in France dedicated to biological materials. It includes three spectrometers operating at temperatures from ca 2 K to 300 K in the pressence of magnetic fields from a few milliteslas up to 8 teslas, applied either parallel or perpendicular to the γ-ray beam.

Applications
Mössbauer spectroscopy allows one to characterize the active sites of iron proteins and to study their functioning. A detailed description of the electronic structure of iron centers can be reached through experiments under applied magnetic fields. Mechanistic studies of iron enzymes can be done by coupling Mössbauer spectroscopy to the rapid-mix freeze-quench technique. Usual studies are performed on isolated proteins, but experiments can be realized on cells or cells components so as to analyze global behaviors in in vivo conditions.

Publications

Arragain S, Garcia-Serres R, Blondin G, Douki T, Clemancey M, Latour JM, Forouhar F, Neely H, Montelione GT, Hunt JF, Mulliez E, Fontecave M and Atta M
Post-translational modification of ribosomal proteins: Structural and functional characterization of RimO from Thermotoga maritima, a radical-SAM methylthiotransferase.
Journal of Biological Chemistry, 2010, 285(8): 5792-5801

Murali M, Nayak S, Costa JS, Ribas J, Mutikainen I, Turpeinen U, Clemancey M, Garcia-Serres R, Latour JM, Gamez P, Blondin G and Reedijk J
Discrete tetrairon(III) cluster exhibiting a square-planar Fe₄(μ₄-O) core: Structural and magnetic properties.
Inorganic Chemistry, 2010, 49(5): 2427-2434

Seguin A, Sutak R, Anne Laure B, Garcia-Serres R, Oddou J-L, Lefevre S, Santos R, Dancis A, Camadro J-M, Latour J-M and Lesuisse E
Evidence that yeast frataxin is not an iron storage protein in vivo.
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2010, 1802(6): 531-538

Horner O, Mouesca JM, Solari PL, Orio M, Oddou JL, Bonville P and Jouve HM
Spectroscopic description of an usual protonated ferryl species in the catalase from Proteus mirabilis and density functional theory calculations on related models. Consequences for the ferryl protonation state in catalase, peroxidase and chloroperoxidase.
Journal of Biological Inorganic Chemistry, 2007, 12(4): 509-525

Mathevon C, Pierrel F, Oddou JL, Garcia-Serres R, Blondin G, Latour JM, Menage S, Gambarelli S, Fontecave M and Atta M
tRNA-modifying MiaE protein from Salmonella typhimurium is a nonheme diiron monooxygenase.
Proceedings of the National Academy of Sciences USA, 2007, 104(33): 13295-13300

Horner O, Oddou J L, Mouesca J M and Jouve H M
Mössbauer identification of a protonated ferryl species in catalase from Proteus mirabilis: Density functional calculations on related models.
Journal of Inorganic Biochemistry, 2006, 100(4): 477-479

Bienkowski K, Kulszewicz-Bajer F, Genoud F, Oddou JL and Pron A
Mixed doping of polyaniline with iron(III) chloride in the presence of hexafluoroacetylacetone: Chemical and structural consequences.
Mat. Chem. Phys., 2005, 92: 27-32

Buga K, Majkowska A, Pokrop R, Zagorska M, Djurado D, Pron A, Oddou JL and Lefrant S
Postpolymerization grafting of aniline tetramer on polythiophene chain: Structural organization of the product and its electrochemical and spectroelectrochemical properties.
Chem. Mater, 2005, 17: 5754-5762

Martinho M, Banse F, Bartoli JF, Mattioli TA, Battioni P, Horner O, Bourcier S and Girerd JJ
New example of a non-heme mononuclear iron(IV) oxo complex. Spectroscopic data and oxidation activity.
Inorganic Chemistry, 2005, 44(25): 9592-9596

Horner O, Mouesca JM, Oddou JL, Jeandey C, Niviere V, Mattioli TA, Mathe C, Fontecave M, Maldivi P,Bonville P, Halfen JA and Latour JM
Mössbauer characterization of an unusual high-spin side-on peroxo-Fe³⁺ species in the active site of superoxide reductase from Desulfoarculus baarsii. Density functional calculations on related models.
Biochemistry, 2004, 43: 8815-8825

d’Autreaux B, Horner O, Oddou JL, Jeandey C, Gambarelli S, Berthomieu C, Latour JM and Michaud-Soret I
Spectroscopic description of the two nitrosyl-iron complexes responsible for Fur inhition by nitric oxide.
Journal of the American Chemical Society, 2004, 126: 6005-6016