The LSOD includes researchers characterizing the molecular mechanisms of stress-related proteins (hemoproteins, flavoproteins and metalloproteins) or detoxification (cytochromes P450, glutathione S-transferase, P-glycoprotein), using biochemical, enzymological, biophysical and bioinformatics techniques.
● Molecular biology for the production of native and mutant proteins (heterologous expression systems: Escherichia coli, Lactococcus lactis).
● Biochemical methods of production, identification and characterization of proteins and / or protein domains, substrates and metabolites.
● Contribution from chemistry: synthetic and biomimetic models (heme and non heme, copper and manganese complexes).
● Raman, resonance Raman and FTIR techniques for characterizing the structure and environment of the chromophores (heme, flavins, ...) studied.
● Electron Paramagnetic Resonance spectroscopies (EPR, ENDOR, ESEEM, HYSCORE) that allow the structural characterization of paramagnetic species (radicals, CuII, FeIII, ...).
● Rapid kinetics (stopped-flow, freeze-quench) and catalytic production (fluorimetry, HPLC ...) methods.
● Molecular modelling methods.