Direction des sciences du vivant

Human resources

Yves BOULARD, Researcher

Jean-Baptiste CHARBONNIER, Researcher

Pascal DREVET, Researcher

Raphaël GUÉROIS, Researcher

Marie-Hélène LE DU, Researcher

Simona MIRON, Researcher

Françoise OCHSENBEIN, Researcher

Maïté PATERNOSTRE, Researcher

Sophie ZINN-JUSTIN, Researcher

Gwenaëlle MOAL-RAISIN, Research Technician

Carine TELLIER-LEBEGUE, Research Technician

Yann-Vaï LE BIHAN, Postdoctoral Fellow

Stéphanie RAMBOARINA, Postdoctoral Fellow

Jeremy AMRAN, PhD Student

Jessica ANDREANI, PhD Student

Benjamin BOURGEOIS, PhD Student

Isaline HERRADA, PhD Student

Nicolas RICHET, PhD Student

The Laboratory of Structural Biology and Radiobiology (LBSR) studies the 3D structure of proteins involved in the structural organization and genomic integrity of the cell nucleus. The various topics developed around this theme have the common aim of describing protein/protein interaction networks involved in biological functions essential for cell survival such as signaling and repair of DNA damages. The target proteins are modular: they include well-folded fragments connected by flexible segments. The globular modules or domains have multiple functions including regulation of the enzymatic activities of proteins and orchestration of the cellular signaling. They are usually conserved during evolution, and we identify them using bioinformatics approaches. Their 3D structure is determined by NMR or X-ray crystallography. Their arrangement within the modular protein is observed by SAXS and NMR. Their interactions are characterized by NMR, biochemistry, fluorescence or calorimetry. Crystallography provides access to the structure of multi-functional protein complexes. Our structures are positionned in electron microscopy maps in order to build pseudo-atomic models of functionally important complexes. Clearly, molecular modeling is a central tool in the laboratory that gives access to models consistent with heterogeneous structural data. On the experimental point of view, in addition to standard laboratory equipment, we have a comprehensive NMR park (500MHz, 600MHz, 700MHz), and we benefit from the proximity of the synchrotron SOLEIL which is in operation since 2006. The laboratory has an extensive expertise in the different techniques that give access to an atomic understanding of biological processes. Its location in an environment of cell biologists and geneticists favors synergies between in vitro and in vivo approaches.

Key words

Structural biology, Cristallography, Nuclear Magnetic Resonance, SAXS, Molecular Modelling, DNA Damage Repair, Signaling, Double-Strand Break, Non Homologous End-Joining, Nuclear Envelope, Telomeres, Histone chaperones, Chromatin assembly, Inhibition of protein-protein interaction, Peptido-mimetics, Genetic Diseases.