Presentation
The goals of the Laboratory "Regulation of Gene Expression and Epigenetic" are to study at the molecular level the mechanisms of gene expression and its control in eukaryotic cells. The approaches employed are biochemical, molecular biology, genetic, cell biological, and genome wide analyses (DNA chips, ChIP, ChIP on Chip) by using yeast and mouse as modele organisms. The combination of these approaches enables the dissection of complex processes of the expression pathway.
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In eukaryotic cells, the genetic information encodes into DNA is transcribed by three transcriptional machineries that constitute one of the most complexe system of the cell. The biochemical and genetic characterization of these complexes in the yeast Saccharomyces cerevisiae are important goals of the laboratory. In addition, each transcriptional machinery is regulated at different levels in order to adapt the cell growth to environmental conditions, and in particular to stresses. The laboratory is currently investigating the regulation networks exerted at the level of these three transcriptional machineries.
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In Eukaryotes, DNA is organized into chromatin whose unit is constituted by the nucleosome. In the last decade, the fundamental role of the chromatine structure in the regulation of gene expression has been unveiled, and in particular of the post-translational modifications of histones. One of the research groups of the unit is interested in unravelling how specific regulatory proteins contribute to genome plasticity , and to the control of gene expression in embryonic stem cells. |
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Olivier LEFEBVRE