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Institutes/ Institute of Biology and Technology Saclay (iBiTec-S)/ Units/ Integrative Biology and Molecular Genetics (SBiGeM)/ Laboratory of DNA metabolism and responses to genotoxic stress (LMARGe)/ Mechanisms of DNA checkpoints (MC. Marsolier-Kergoat)

Mechanisms of DNA checkpoints

Marie-Claude MARSOLIER-KERGOAT
CEA Saclay/Bât. 144

Tél: 01 69 08 83 54
mcmk@cea.fr


iBiTec-S / SBIGeM / LMARGe


Human resources
Arach GOLDAR, Researcher
François LETEURTRE, Researcher
Marie-Claude MARSOLIER-KERGOAT, Group Leader
Anne PEYROCHE, Researcher
Marie-Bénédicte BARRAULT, Research Engineer
Thomas LEJEUNE, Research Technician
Chloe GODARD, Research Technician CDD
Ilektra KOURANTI, Postdoctoral Fellow
Julie MEURISSE, PhD Student
Céline PELLENTZ, PhD Student



Research Programs

Our research interests have in common their relationships to the DNA checkpoints, which are response pathways activated by DNA lesions or the stalling of replication forks. These checkpoints control several responses including cell cycle blocks, activation of the ribonucleotide reductase, post-translational modifications of histones or repair proteins, which increase cell survival and reduce the fixation of mutations. Many cancers, either hereditary or sporadic, are accompanied by mutations in checkpoint genes, hence their designation as "anti-cancer barriers".

    Our research activities concern DNA checkpoint mechanisms in the yeast Saccharomyces cerevisiae (MC. Marsolier-Kergoat), and in mammal cells (F. Leteurtre) and also include a series of related themes such as studies about the proteasome (A. Peyroche) and the analysis of DNA replication by experimental (DNA combing) and theoretical approaches (computer simulations) (A. Goldar).


Key words
DNA checkpoints, DNA replication, proteasome, yeast, mammal cells.


Publications

Barrault MB, Richet N, Godard C, Murciano B, Le Tallec B, Rousseau E, Legrand P, Charbonnier JB, Le Du MH, Guérois R, Ochsenbein F, Peyroche A. (2012). Dual functions of the Hsm3 protein in chaperoning and scaffolding regulatory particle subunits during the proteasome assembly. Proc Natl Acad Sci USA (Sous Presse).

Ma E, Hyrien O, Goldar A.  (2012). Do replication forks control late origin firing in Saccharomyces cerevisiae? Nucleic Acids Res. 40, 2010-2019.

Boutin C, Stopin A, Lenda F, Brotin T, Dutasta J P, Jamin N, Sanson A, Boulard Y, Leteurtre F, Huber G, Bogaert-Buchmann A, Tassali N, Desvaux H, Carriere M, Berthault P.  (2011). Cell uptake of a biosensor detected by hyperpolarized Xe-129 NMR: The transferrin case. Bioorg Med Chem. 19, 4135-4143.

Guilbaud G, Rappailles A, Baker A, Chen C L, Arneodo A, Goldar A, d'Aubenton-Carafa Y, Thermes C, Audit B, Hyrien O.  (2011). Evidence for Sequential and Increasing Activation of Replication Origins along Replication Timing Gradients in the Human Genome. PLoS Comput Biol. 7, 22.

Ma E, Goldar A, Verbavatz J M, Marsolier-Kergoat M C.  (2011). Giant yeast cells with nonrecyclable ribonucleotide reductase. Mol Genet Genomics. 285, 415-425.

Marsolier-Kergoat M C.  (2011). A simple model for the influence of meiotic conversion tracts on GC content. PLoS ONE. 6, e16109.

Aucher W, Becker E, Ma E, Miron S, Martel A, Ochsenbein F, Marsolier-Kergoat MC, Guérois R.  (2010). A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data. Mol Cell Proteomics. 9, 42.

Hyrien O, Goldar A. (2010). Mathematical modelling of eukaryotic DNA replication. Chromosome Res., 18, 147-161.

Clémenson C, Marsolier-Kergoat MC.  (2009). DNA damage checkpoint inactivation: Adaptation and recovery. DNA Repair. 8, 1101-1109.

Goldar A, Marsolier-Kergoat MC, Hyrien O.  (2009). Universal temporal profile of replication origin activation in eukaryotes. PLoS ONE. 4, e5899.

Le Tallec B, Barrault MB, Guérois R, Carré T, Peyroche A. (2009). Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome. Mol Cell. 33, 389-399.

Marheineke K, Goldar A, Krude T, Hyrien O. (2009). Use of DNA combing to study DNA replication in Xenopus and human cell-free systems. Methods Mol. Biol., 521, 575-603.

Marsolier-Kergoat MC, Yermian E.  (2009). GC Content and Recombination: Reassessing the Causal Effects for the Saccharomyces cerevisiae Genome. Genetics. 183, 31-38.

Baldwin GS, Brooks NJ, Robson RE, Wynveen A, Goldar A, Leikin S, Seddon JM, Kornyshev AA.  (2008). DNA double helices recognize mutual sequence homology in a protein free environment. J Phys Chem B. 112, 1060-4.

Douarche C, Sikorav JL, Goldar A. (2008).Aggregation and adsorption at the air-water interface of bacteriophage {varphi}X174 single-stranded DNA. Biophys J. 94: 134-146.

Goldar A, Labit H, Marheineke K, Hyrien O.  (2008). A dynamic stochastic model for DNA replication initiation in early embryos. PLoS ONE. 3, e2919.

Goldar A, Thomson H, Seddon JM.  (2008). Structure of DNA cholesteric spherulitic droplet dispersions. J Phys Condens Mat. 20, 035102.

Labit H, Goldar A, Guilbaud G, Douarche C, Hyrien O, Marheineke K.  (2008). A simple and optimized method of producing silanized surfaces for FISH and replication mapping on combed DNA fibers. Biotechniques. 45, 649-.

Mergui X, Leteurtre F, Lipinski M, Bénard J, Amor-Guéret M.  (2008). Two distinctly altered cellular responses to DNA double-strand breaks in human neuroblastoma. Biochimie. 90, 1656-66.

Camier S, Ma E, Leroy C, Pruvost A, Toledano M, Marsolier-Kergoat MC.  (2007). Visualization of ribonucleotide reductase catalytic oxidation establishes thioredoxins as its major reductants in yeast. Free Radic Biol Med. 42, 1008-10016.

Douarche C, Sikorav JL, Goldar A. (2007). Aggregation and adsorption at the air-water interface of bacteriophage {varphi}X174 single-stranded DNA. Biophys J. 94, 134-46.

Guillemain G, Ma E, Mauger S, Miron S, Thai R, Guérois R, Ochsenbein F, Marsolier-Kergoat MC.  (2007). Mechanisms of checkpoint kinase Rad53 inactivation after a double-strand break in Saccharomyces cerevisiae. Mol Cell Biol. 27, 3378-3389.

Lancelot N, Charier G, Couprie J, Duband-Goulet I, Alpha-Bazin B, Quémeneur E, Ma E, Marsolier-Kergoat MC, Ropars V, Charbonnier JB, Miron S, Craescu CT, Callebaut I, Gilquin B, Zinn-Justin S. (2007). The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA. Nucleic Acids Res. 35, 5898-912
 
Le Tallec B, Barrault MB, Courbeyrette R, Guérois R, Marsolier-Kergoat MC, Peyroche A. (2007). 20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals. Mol Cell 27, 660-74.

Oliva-Trastoy M, Berthonaud V, Chevalier A, Ducrot C, Marsolier-Kergoat MC, Mann C, Leteurtre F.  (2007). The Wip1 phosphatase (PPM1D) antagonizes activation of the Chk2 tumour suppressor kinase. Oncogene. 26, 1449-1458.

Clémenson C, Marsolier-Kergoat MC.  (2006). The Spindle Assembly Checkpoint Regulates the Phosphorylation State of a Subset of DNA Checkpoint Proteins in Saccharomyces cerevisiae. Mol Cell Biol. 26, 9149-9161.

Coulomb S, Bauer M, Bernard D, Marsolier-Kergoat MC.  (2005). Gene essentiality and the topology of protein interaction networks. Proc Biol Sci. 272, 1721-1725.

Chantalat S, Park S, KHua ZL, Liu K, Gobin R, Peyroche A, Rambourg A, Graham TR, Jackson CL.  (2004). The Arf activator Gea2p and the P-type ATPase Drs2p interact at the golgi in Saccharomyces cerevisiae. J Cell Sci. 117, 711-722.

Goldar A, Sikorav JL.  (2004). DNA renaturation at the water-phenol interface. Eur Phys J E. 14, 211-239

Chantalat S, Courbeyrette R, Senic-Matuglia F, Jackson CL, Goud B, Peyroche A.  (2003). A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p. Mol Biol Cell. 14, 2357-2371.

Leroy C, Lee SE, Vaze MB, Ochsenbein F, Guérois R, Haber JE, Marsolier-Kergoat MC.  (2003). PP2C Phosphatases Ptc2 and Ptc3 Are Required for DNA Checkpoint Inactivation after a Double-Strand Break. Mol Cell. 11, 827-835.

Li X, Leteurtre F, Rocha V, Guardiola P, Berger R, Daniel MT, Noguera MH, Maarek O, Roux GL, De La Salmoniere P, Richard P, Gluckman E.  (2003). Abnormal telomere metabolism in Fanconi's anaemia correlates with genomic instability and the probability of developing severe aplastic anaemia. Br J Haematol. 120, 836-845.

Perez MD, Dubner D, Michelin S, Leteurtre F, Carosella ED, Gisone PA.  (2002). Radiation-induced up-regulation of telomerase in KG1a cells is influenced by dose-rate and radiation quality. Int J Radiat Biol. 78, 1175-1183.