Department of genetic instability, recombination and repair (SIGR)
The LRIG, the LMR, the LRD and the LERA together form a joint CEA-CNRS unit.
Laboratory of DNA radiobiology (LRD)
Molecular biology and genetics, protein biochemistry: expression and purification
Study of the molecular mechanisms of radiation damaged DNA repair.
Analysis of the biological, mutagenic and cancerogenic consequences of radiation.
Study of the molecular mechanisms of radiation damaged DNA repair.
Analysis of the biological, mutagenic and cancerogenic consequences of radiation.
Laboratoire d'études de la réparation de l'ADN (LERA)
Genome stability is maintained throughout successive divisions by the controlling action of a certain number of proteins, among which the Sgs1 and Srs2 helicases, DNA damage checkpoint proteins as well as polymerases and their ancillary factors.
We are studying the mechanisms that ensure genome perennity during replication by preventing the occurrence of deleterious recombination events. We are also analyzing factors that condition the repair mode of spontaneous (or exogenously induced) damage occurring during the progression of the replication fork. Our approaches combine formal and molecular genetics, electron microscopy, biochemistry and enzymology.
We are studying the mechanisms that ensure genome perennity during replication by preventing the occurrence of deleterious recombination events. We are also analyzing factors that condition the repair mode of spontaneous (or exogenously induced) damage occurring during the progression of the replication fork. Our approaches combine formal and molecular genetics, electron microscopy, biochemistry and enzymology.
Telomeres and Chromosome Repair Laboratory (LTR)
Telomeres, the DNA-protein complexes at the ends of linear chromosomes, normally protect the native chromosome ends from the DNA damage repair and checkpoint pathways that act on ends generated by double-strand breaks. Telomere failure can lead to chromosome fusions and the appearance of dicentric chromosomes. Dicentrics breakage during mitosis causes further DNA damage and rearrangements that result in cell death or mutagenesis through genes loss and amplification, promoting oncogenesis.
Laboratory for Research in Genetic Instability (LRIG)
Molecular mechanisms of DNA repair in mammals, in particular oxidative damage : oxidized bases, abasic sites, strand breaks. Purification of DNA repair proteins, biochemical studies of enzymatic activities. Cellular aspects of DNA repair, response of repair systems, protein relocalization as a result of oxidative stress (confocal microscopy). Effect of Cd on DNA repair proteins.
Role of DNA repair mechanisms on the genetic variability of the pathogenic bacterium Helicobacter pylori. Bacterial genetics, recombination enzyme biochemistry. Virulence effects.
Role of DNA repair mechanisms on the genetic variability of the pathogenic bacterium Helicobacter pylori. Bacterial genetics, recombination enzyme biochemistry. Virulence effects.