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Institutes/ Institute of environmental biology and biotechnology (IBEB)/ Services iBEB/ Department of plant biology and environmental microbiology (SBVME)/ Research group in applied phytotechnics (GRAP)/ The platform/ The IBISA platform

The IBISA platform


The GRAP is a multi-field team (biologists and technologists) which manages, develops and ensures technical maintenance of a platform dedicated to the production of plants in controlled conditions. This platform accommodates the experiments of various laboratories of the Department, in particular within the framework of programs study of the biological effects of heavy metals and of radionucleides, and the biological effects of the oxidizing stresses on plants, as well as studies on photosynthesis. Opened on exterior, it also allows to undertake programs in collaboration with other institutes or to carry out services within the framework of contracts with industrialists or agencies of program (European Community, Ministries,…). It is identified as a technological platform for plant studies by the GIS IBISA.

GIS IBISA

The platform consists of two principal entities:

  • A park of 16 cabinets of variable volumes (from a few liters to 1500 liters) allowing the culture of plants under completely controlled conditions. They are hermetically closed modules in which the whole of the parameters of the environment are measured and controlled finely permanently. The level of light (until approximately 2000 µmol photons.m-2.s-1), the temperature (of 1 with 50°C), the relative humidity (from 20 to 80%), the nutrient alimentation (qualitative and quantitative regulation), the composition of the gas atmospheres (N2, CO2, O2, contributions of other gases, modifications of the isotopic abundance,…) are monitored during complete cycles of growth of plants being able to reach several months. Moreover gaseous exchange between the plants and their environment (photosynthesis, respiration, transpiration) can there be measured permanently. This unit is controlled by a system of control-command ensuring a made safe operation, supervised by data processing.

 

  • Nine phytotrons (growth chambers in which essential parameters for the production of plants - light, temperature, relative humidity and mineral nutrient -  are controlled) and two in vitro chambers for GMO culture (Genetically Modified Organisms). This installation is approved for the culture class 2 GMO (in particular Arabidopsis thaliana). It offers an useful surface area of about 70 m2 allowing the production of about 20 000 Arabidopsis plants. Two other phytotrons are specifically dedicated to experiments using toxics and/or radionucleides.

  • Two phytotrons (in the “Salto” facilities, a classified area) are dedicated to experiments with toxic elements (radioactive or stable).



You can download here the brochure of the plateform (PDF)

For any information please contact Michel Péan: (+33) 4 42 25 71 85 - michel.pean@cea.fr