Combinatorial chemistry and high throughput screening laboratory
- Center of Bio-Active Molecules Screening
- Combinatorial chemistry and high throughput screening laboratory
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| Missions . Speed-up bio-active compounds discovery process . Use bio-active compounds as chemical tools for biological investigations.
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. High-throughput screening of commercial and "in-house" chemical libraries. . Identification of compounds ("hits") that modulate a biological target. . Optimization (analogue synthesis) using solid and/or liquid phase combinatorial chemistry. |
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. "In-house" chemists can speed-up (1) bio-active compounds optimization and (2) development and preparation of specific chemical libraries. . Radioactive-based and/or cell-based high-throughput screening (3H, 14C, 125I, ...). . Radiolabeled compound synthesis (3H, 14C, 125I, ...). Combining combinatorial chemistry and high-throughput screening, this platform is dedicated to the research programmes in radiobiology and nuclear toxicology. |
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. 4 staff members and 2 graduate students. . A combinatorial chemistry laboratory: HPLC-MS, parallel synthesis robot, solid phase synthesis robot, balance automator, and specific combichem devices. . A high-throughput screening workstation in a sterile environment along with a cell culture room and a microbiology room is planned for 2003 (2 000 compounds tested/day): Robotic station including a liquid handling system and an articulated arm, CO2 incubator, microplate washer, fluorescence–luminescence–TRF microplate reader, monochromator UV–VIS microplate reader (200–1000 nm) radioactive microplate reader (ß and gamma). . 20 000 drug-like compounds library. |
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Contacts:
(CEA Saclay, IBIEC-S, SCMB): Roman Lopez, Bernard Rousseau roman.lopez@cea.fr |
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Adonis Création - crédit photo : © CEA, ©CEA/P Dumas , ©CEA/Y Ambroise