Version française
Life Science Div./ Technology platforms/ Biochemistry for post-genomics / Biochemistry for post-genomics

Biochemistry for post-genomics


A post-genomics biochemistry platform in Marcoule, which ensures high output production of candidate proteins for functional or structural studies

PDF version (117.83 kB)
Goal: The identification of proteins as new molecular targets of drugs or toxic compounds takes advantage of the recent advances in genome sequencing. Bioinformatics, transcriptomics and proteomics are used for the high throughput discovery of novel proteins and pathways. In this respect, a "new" biochemistry for post-genomics is needed to obtain practically these targets for functional or structural studies, opening a large field of biotechnological applications.
 
 
What we do
Our biochemical approaches are integrated "from sequence to purified protein" and are developed upon mature or innovative techniques for:
. production of  recombinantproteins
(genetic engineering, overexpression in E.coli, yeasts, CHO, COS, Sf9 / baculovirus…, biomass production in 60 liters tanks)		   . purification of native and recombinant proteins,
their folding, and their characterization by biophysical techniques.		   . large scale production of, polyclonal and monoclonal antibodies
 
Specific strategies are developed depending on the choice of high purity, or large number, or high volume of; proteins to be produced. The laboratory is focusing on "difficult" proteins, that means membrane proteins, modular proteins, heteromeric complexes as well as post-translationally modified proteins.

  . Collaborations
The laboratory of post-genomics and nuclear toxicology promotes research projects in collaboration with public laboratories and with R&D industrial companies.
It is labelled ISO 9001:2000 for its research project management in protein biochemistry. In addition, it participates to local organizations of incubators and support to biotechnological start-ups.

 
Contacts:
DSV, CEA/Marcoule, 30207 Bagnols sur Cèze cédex.
Eric QUEMENEUR, Service de Biochimie
post-génomique et Toxicologie Nucléaire


Adonis Création - credit photo : ©CEA/C Dupont