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Life Science Div./ Technology platforms/ Platform for biochips/ Platform for biochips/ Phenotypic analysis on a chip

Phenotypic analysis on a chip

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Miniaturised Technologies for phenotypic cell analysis:

Confinement of cell cultures in liquid drops is an important step for undertaking multiple tests in cells. With the system developed and shown here, it is possible to deposit up to 1,000 drops of cell cultures, each one containing approximately one hundred cells on one histological plate (15 cm2) representing 1,000 truly independent experiments!
DropChip:
formation of 100 nl drops on 400 spots with the piezoelectric nozzle.
Multi-phenotypic image capture in a U373 cells plot (500 µm):
• cell nucleus (Hoescht - in blue)
• cell death (“DeadRed” - in red)
• cell survival (SYTO10 - in green)


Phenotypes detecttion and quantification:

1. Using fluorescence (collaboration with Imstar):
mosaic presentation of 12 U373 cell spots of the DropChip (cell stained with SYTO10 and fixed with PFA on the chip).		  
3.Biostatistic and database

2. Using mass spectrometriy:



 
 
 
Innovation Validations:
These chips are developed and used at CEA in oncology and toxicology frameworks. Two projects are run to assess their performance:
  • iCancéroDrops (granted for by Ligue contre le Cancer): efficiency improvement of anticancer drugs by specifically blocking the expression of interesting genes using siRNAs. Within this project, chips provide the support for an accurate analysis of cell behaviour in 40,000 reactions inside nano-drops where the following conditions change: cell type, anticancer drug concentration, concentration and type of siRNAs and phenotype studied.
  • Toxdrop (paid for by European Community): making use of cell chips in order to substitute multi-parameter cytotoxicity analyses to animal testing.
 
 
Collaborations:
  • To increase the analytical quality, we take part in the development of microscopic tools, within a collaboration with company Imstar.
  • Mass spectroscopy analysis of cell behaviour within drops and modelling: collaboration with CHU INSERM 318 in Grenoble and company Tascon.
  • Information provided are collected, organised and analysed using bio-information tools (collaboration with Y. Vandenbrouck team of CEA-DRDC in Grenoble).
  • Cell toxicology: INSERM U 522 and CNRS UMR 5534
  • Cell oncology: FRE 2692 CNRS, INSERM EMI 104, and INSERM U 318




 
Contacts:
Life Sciences Division: F. Chatelain (Francois.Chatelain@cea.fr)
Leti: P. Puget


Adonis Création - crédit photo: ©CEA